info@biolink.com
0086-400-058-9000
CN
Products
Application & Solutions
Single-Use Bioprocess Solutions Monoclonal Antibody Production Vaccine Production mRNA Production
Resource
Application Note White Paper
Support
FAQ Validation Service
Company
About Us Facilities Career Company News
Contact Us
Search
×
Single-use Bioprocess Products Cell Culture Products Protein Chromatography Processing Filtration Hydration Products
Single-Use Bioprocess Bags & Bottles Single-Use Mixing System Aseptic Connect & Disconnect Aseptic Sampling & Filling Bioprocess Supporting Vessels Connectors and Fittings
BioHub® 2D Single-use Storage Bag BioHub® 3D Single-use Storage Bag BioHub® Single-use Freeze-thaw Bag NovaLinX® Nuclease-free Single-use Consumables HemoLinX® CFT Freezing Bag BioHub® Single-use Storage Bottle BioHub® Storage Bottle Tool Kit BioHub® BM Single-use Magnetic Mixing System BioHub® DS Single-use Desktop Mixing System BioHub® UM Top-driven Mixing System BioHub® FD Feeding System BioHub® Single-use Powder-feeding Bag BioHub® MB Single-use Mixing Bag BioHub® Single-use Weighing Bag BioHub® Sealer Ⅱ BioHub® Handheld Sealer BioHub® Welder Ⅱ BioHub® Sterile Cutter BioHub® Single-use Sampling Bag BioHub® Single-use Sampling Bottle BioHub® Sterile Water Sampling Bag BioHub® S Sterile Sampling Tray BioHub® Single-use Filling System BioHub® Fill Single-use Bulk Filling System Circular Plastic Bin Plastic Freeze-thaw Box Cubic Foldable Plastic Bin Cubic Stainless Steel Tank Storage Tray Storage Tray Transfer Cart BioHub® Connectors BioHub® Sanitary Clamp Fittings Biohub® Hose Plug BioHub® Diaphragm Gasket BioHub® Clamp & End Cap BioHub® Integrated Diaphragm Gauge Tee SynaLinX® B-Flex™ Thermoplastic Tubing SynaLinX® TST Platinum Cured Silicone Tubing SynaLinX® HST Platinum Cured Silicone Tubing CytoLinX® RW 10-200L Single-use Rocking Bioreactor CytoLinX® GB 1-20L Benchtop Glass Bioreactor CytoLinX® BR 10-2000L Single-use Bioreactor CytoLinX® WB Single-use Cell Culture Bag CytoLinX® WB Single-use Cell Therapy Bag CytoLinX® Single-use Breathable Cell Bag CytoLinX® BRTF Single-use Top-driven Bioreactor Bag BioHub® Magnetic Separator BioHub® Automated Bioprocess Analyzer Puredex® Cyto-1 Microcarrier Chrom-LinX® Lab-scale Empty Chromatography Column Chrom-LinX® Manual Compression Chromatography Column Chrom-LinX® Automated Chromatography Column Chrom-LinX® Auto TH Chromatography Column PuriLinX® Benchtop Chromatography System VERDOT Ips² InPlace™ Process-scale Chromatography Column ChromaX® Geldex® Prepacked Column Chrom-Trap® & Chrom-Screen™ Prepacked Column ChromaX® A20 Affinity Chromatography Column Chromstar® FF Resin for Size Exclusion Chromatography Chromstar® CL Resin for Size Exclusion Chromatography Chromstar® B Resin for Size Exclusion Chromatography Geldex® PG Resin for Size Exclusion Chromatography Puredex® G Resin for Size Exclusion Chromatography Puredex® LH-20 Resin for Size Exclusion Chromatography Maxtar® Resins for Ion Exchange Chromatography Maxtar® HR Resins for Ion Exchange Chromatography Chromstar® XL Resins for Ion Exchange Chromatography Chromstar® BB Resins for Ion Exchange Chromatography Chromstar® FF Resins for Ion Exchange Chromatography Chromstar® HP Resins for Ion Exchange Chromatography Chromstar® XL Resins for Ion Exchange Chromatography Puredex® Resins for Ion Exchange Chromatography MaXtar® ARPA Affinity Chromatography Resins MaXtar® rProtein A Affinity Chromatography Resins MaXtar® Protein G Affinity Chromatography Resins rProtein A Chromstar® FF Affinity Chromatography Resins Protein G Chromstar® 4FF Affinity Chromatography Resins Ni Chromstar® Affinity Chromatography Resins IMAC Chromstar® Affinity Chromatography Resins Chelating Chromstar® FF Affinity Chromatography Resins Glutathione Chromstar® Affinity Chromatography Resins MaXtar® PlasmidCap HR Affinity Chromatography Resins MaXtar® Heparin Affinity Chromatography Resins Heparin Chromstar® Affinity Chromatography Resins Benzamidine Chromstar® 4FF Affinity Chromatography Resins MaXtar® Blue Affinity Chromatography Resins Blue Chromstar® Affinity Chromatography Resins NHS Activated Chromstar® 4FF Affinity Chromatography Resins CNBr Activated Chromstar® Affinity Chromatography Resins MaXtar® Hydrophobic Chromatography Resins MaXtar® HR Hydrophobic Chromatography Resins Chromstar® FF Hydrophobic Chromatography Resins Chromstar® HP Hydrophobic Chromatography Resins MaXtar® MMC Multimodal Chromatography Resins Hydroxyapatite BARONHAP® Type I&II Multimodal Chromatography Resin Maxgo® 50S Maxgo® 30S Maxgo® 15/30RPC MonoPS® Oligo dT (25) Pro Affinity Resin FiltraLinX® Lab TFF System FiltraLinX® Pilot TFF System FiltraLinX® Benchtop Manual Hollow Fiber TFF System FiltraLinX® Benchtop TFF System FiltraLinX® Automatic TFF System FiltraLinX® Manual TFF System FiltraLinX® Semi-automatic TFF System FiltraLinX® Benchtop Manual Cassette TFF System Cernolix® Series KSG Hydrophilic Filters VentiLink® Series ESG Hydrophobic Filters Puruflon® FSG Hydrophobic Filters Cruclix™ Hollow Fiber Filter FiltraLinX® Depth Filter Holder (Type P3C) FiltraLinX® Depth Filter Holder (Type M) FiltraLinX® Virus Filter Holder FiltraLinX® Tangential Flow Filtration Holder HydroLinX® Water for Injection in Bottles HydroLinX® Water for Injection in Bags HydroLinX® Hydration Products HydroLinX® Buffer Solutions and Process Liquids HydroLinX® Antifoam Emulsion
E-mail
temp1
temp1

CytoLinX® BRTF Single-use Top-driven Bioreactor Bag

The core of the CytoLinX® BRTF 50 L microbial fermentation system is the single-use microbial bioreactor bag designed to meet the stringent requirements of microbial fermentation. It is used for cultivating various organisms, including E-coli, pseudomonas, and yeast. The single-use microbial bioreactor bag is based on the proven design and materials of the CytoLinX® BRTF Single-Use Bioreactor Bag for mammalian cell culture.

  • RENOLIT 9101 multilayer co-extruded film, ULDPE liquid contact layer, offering excellent biocompatibility and chemical compatibility while ensuring low levels of extractable content.

  • The dual impeller design enables vigorous mixing of the culture, and the bottom magnetic coupling eliminates external shafts, minimizing the risk of leakage.

  • All single-use microbial bioreactor bags are equipped with pressure sensors to maintain bag integrity during demanding fermentation processes.

  • The vent filter is equipped with a condensation bag at the front end to integrate condensation, returning water vapor from the gas back into the bag, ensuring the ventfilter remains unclogged.

Inquiry
Download
Features Specifications Contact us

Features of CytoLinX® BRTF Single-use Top-driven Bioreactor Bag

CytoLinX® BRTF Single-use Top-driven Bioreactor Bag is designed to match single-use top mechanical coupling bioreactor used in biopharmaceuticals. The product can be used for scientific research, process development and commercial production of CHO, Vero, and MDCK cells, etc.

  • FirmaX R S32 multilayer co-extruded film, ULDPE fluid contact layer, offering excellent biocompatibility and chemical compatibility while ensuring low levels of extractable content 

  • The porous-frit microsparge column is designed from ultra-high molecular weight polyethylene (UHMW- PE), with pore sizes ranging from 20-40 μm. The generated bubbles possess a high surface area ratio and good oxygen transfer. UHMW-PE exhibits outstanding impact resistance, wear resistance, chemical corrosion resistance, physiological inertness, adaptability, and hydrophobicity

  • The macro-perforated microporous membrane is a dispersed aeration disc based on film. Laser-drilled to maintain uniform pore size, various specifications such as 0.178mm, 0.233mm, 0.368mm, 0.445mm, 0.582mm are available, tailored with specific apertures and quantities for each bag specification

  • Equipped with imported filters to ensure bag integrity

  • All pipelines can be flexibly customized

  • Fully validated, complete validation reports can be provided


Specifications of CytoLinX® BRTF Single-use Top-driven Bioreactor Bag

Standard Configuration of CytoLinX® BRTF Single-use Top-driven Bioreactor Bags

Volume

Version

Cat. No.

Configuration

brtf single use top driven bioreactor bags

DHS+Frit

5:1


BRTF-

0050-C221

  • a (top-driven mixing parts): 3-blade impeller, diameter: 111.1 mm, angle: 45°

  • b (overlay): pressure sensor, ESG02MGSHHG12 (Saint-Gobain), 20 cm1/2" x 3/4" TPE thermoplastic tubing + 16 cm 1/4" x 7/16" TPE thermo-plastic tubing

  • c (intlet / feed): 150 cm 1/4" x 7/16" TPE thermoplastic tubing, 30 cm 1/8" x 1/4" TPE thermoplastic tubing plug

  • k (intlet / feeding port): 150 cm 3/8" x 5/8" TPE thermoplastic tubing, Y connector

  • tubing 1: 40 cm 3/8" x 5/8" TPE thermoplastic tubing, plug

  • tubing 2: 10 cm 3/8" x 5/8" TPE thermoplastic tubing, 30 cm 1/4" x 7/16" TPE thermoplastic tubing, plug

  • l (intlet): 180 cm 3/8" x 5/8" TPE thermoplastic tubing, plug

  • n (feeding port): 15 cm 1/4" x 7/16" TPE thermoplastic tubing, 150 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • m (vent filter): 20 cm 1/2" x 3/4 TPE thermoplastic tubing, Y connector

  • tubing 1: 25 cm 1/2" x 3/4" TPE thermoplastic tubing, ESG02MGSHHG12(Saint-Gobain)

    tubing 2: 15 cm 1/2" x 3/4" TPE thermoplastic tubing, AseptiQuik® G sterile connector, 1/2"HB

  • d, e, f, g (sensor): AseptiQuik® G aseptic connector, 1/2"HB

  • i (sampling): thermowell, ID3.5 mm,

    tubing 1: 30 cm 1/8" x 1/4" TPE thermoplastic tubing, needless sampling

    tubing 2: 80 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • d(sensor): thermowell, ID3.5 mm

  • j (harvest port): 100 cm 1/2" x 3/4" TPE thermoplastic tubing, 30 cm 3/8" x 5/8" 1/8" x 1/4" TPE thermoplastic tubing, plug

  • o, p (bottom gas):15 cm 1/4" x 7/16" TPE thermoplastic tubing, check valve, 150 cm 1/4" x 7/16" TPE thermoplastic tubing, ESG02MGSHHG12(Saint-Gobain)

DHS+Frit

2:1


BRTF-

0050-C222



Volume

Version

Cat. No.

Configuration

CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag

DHS+Frit

5:1

BRTF-

0250-C221

  • a (top-driven mixing parts): 3-blade impeller, diameter: 200 mm, angle: 45°

  • i (overlay): pressure sensor,ESG02MGSTKG12 (Saint-Gobain),

    20 cm1/2" x 3/4"TPE thermoplastic tubing+ 16 cm 1/4" x 7/16"TPE thermoplastic tubing

  • m (feeding port): 15 cm 1/4" x 7/16"TPE thermoplastic tubing, 180 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • k, l (intlet/feeding port): 150 cm 3/8" x 5/8" TPE thermoplastic tubing, Y connector,

  • tubing 1: 30 cm 3/8" x 5/8" TPE thermoplastic tubing, plug;

  • tubing 2: 20 cm 3/8" x 5/8" TPE thermoplastic tubing, 40 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • n (intlet/feeding port): 150 cm 1/4" x 7/16" TPE thermoplastic tubing, 50 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • j (vent filter): 42 cm 3/4" x 1", TPE thermoplastic tubing, Y connector,

    tubing 1: 30 cm 1/2" x 3/4" TPE thermoplastic tubing, ESG02MGSHHG12(Saint-Gobain)

    tubing 2: 20 cm 1" x 3/4" TPE thermoplastic tubing, AseptiQuik® G sterile connector, 1/2"HB

  • b, c, d, e (sensor): AseptiQuik® G aseptic connector, 1/2"HB

  • g (sampling port): 10 cm 1/8" x 1/4" TPE thermoplastic tubing, Y-connector,


    tubing 1: 30 cm 1/8" x 1/4" TPE thermoplastic tubing, needless sam-pling

    tubing 2 : 80 cm 1/8" x 1/4"  TPE thermoplastic tubing, plug

  • f (sensor): thermowell, ID3.5 mm

  • h (harvest port): 150 cm 1/2" x 3/4" TPE thermoplastic tubing, plug

  • o, p (bottom gas): ESG02MGSTKG12, 15 cm 1/4" x 7/16" TPE thermoplastic tubing, check valve, 150 cm 1/4" x 7/16"  TPE thermoplastic tubing

DHS+Frit

2:1

BRTF-

0250-C222

CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag

DHS+Frit

5:1

BRTF-

0500-C221

  • a (top-driven mixing parts): 3-blade impeller, diameter: 251 mm, angle: 45°

  • k (overlay): pressure sensor,ESG02MGSTKG12 (Saint-Gobain),

    25 cm 1/2" x 3/4" TPE thermoplastic tubing + 16 cm 1/4" x 7/16"TPE thermoplastic tubing

  • l (feeding port): 15 cm 1/4" x 7/16" TPE thermoplastic tubing, 230 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • m (intlet/feeding port): 250 cm 1/4" x 7/16" TPE thermoplastic tubing, plug,

  • n, o (intlet/feeding port): 180 cm 3/8" x 5/8" TPE thermoplastic tubing, Y-connector

  • tubing 1: 30 cm 3/8" x 5/8"TPE thermoplastic tubing, plug;

  • tubing 2: 30 cm 3/8" x 5/8" TPE thermoplastic tubing, 30 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • p (intlet/feeding port): 250 cm 3/8" x 5/8" TPE thermoplastic tubing, plug

  • j (vent filter): 40 cm 3/4" x 1" TPE thermoplastic tubing, Y-connector

    tubing 1: 30 cm 3/4" x 1" TPE thermoplastic tubing, ESG02MGETTG12(Saint-Gobain)

    tubing 2: 20 cm 3/4" x 1" TPE thermoplastic tubing, AseptiQuik® G sterile connector, 3/4"HB

  • b, c, d, e, f (sensor): AseptiQuik® G sterile connector, 1/2"HB

  • g (sampling): 10 cm 1/8" x 1/4" TPE thermoplastic tubing, needless sampling

    tubing 1: 30 cm 1/8" x 1/4" TPE thermoplastic tubing, needless sampling

    tubing 2: 80 cm 1/8" x 1/4" TPE thermoplastic tubing,plug

  • h (sensor): thermowell, ID3.5 mm

  • i (harvest port): 150 cm 1/2" x 3/4" TPE thermoplastic tubing, plug

  • q, r (bottom gas): ESG02MGSTKG12 (Saint-Gobain), 16 cm 1/4" x 7/16"TPE thermoplastic tubing, 185 cm 1/4" x 7/16" TPE thermoplastic tubing


Volume

Version

Cat. No.

Configuration

CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag

DHS+Frit

5:1

BRTF-

1000-C221

  • a (top-driven mixing parts): 3-blade impeller, diameter: 321 mm, angle: 45°

  • k (overlay): pressure sensor, ESG02MGSTKG12 (Saint-Gobain), 50 cm1/2" x 3/4" TPE thermoplastic tubing + 20 cm 1/4" x 7/16"TPE thermoplastic tubing

  • l (feeding port): 15 cm 1/4" x 7/16" TPE thermoplastic tubing, 250 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • m (intlet/feeding port): 250 cm 1/4" x 7/16"TPE thermoplastic tubing, 30 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • n, o (intlet/feeding port): 250 cm 3/8" x 5/8" TPE thermoplastic tubing, Y-connector,

    tubing 1: 30 cm 3/8" x 5/8" TPE thermoplastic tubing, plug;

    tubing 2: 20 cm 3/8" x 5/8" TPE thermoplastic tubing, 35 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • j (vent filter): ESG02MGETTG12 (Saint-Gobain)×2, Y-connector,

    tubing 1: 25 cm 3/4" x 1" TPE thermoplastic tubing

    tubing 2: 25 cm 3/4" x 1" TPE thermoplastic tubing

  • b, c, d, e, f (sensor): AseptiQuik® G aseptic connector, 1/2"HB

  • g (sampling port): 10 cm 1/8" x 1/4"TPE thermoplastic tubing, Y-connector,

    tubing 1: 30 cm 1/8" x 1/4" TPE thermoplastic tubing, needless

    tubing 2: 80 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • h (sensor): thermowell, ID3.5 mm

  • i (harvest port): 160 cm 1/2" x 3/4"TPE thermoplastic tubing, 30 cm 3/8" x 5/8"TPE thermoplastic tubing, Y- connector

    tubing 1: 30 cm 3/8" x 5/8" TPE thermoplastic tubing, plug;

    tubing 2: 20 cm 3/8" x 5/8"TPE thermoplastic tubing, 35 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • p, q(sparger): ESG02MGSTKG12( Saint-Gobain), 16 cm 1'4" x 7/16" TPE thermoplastic tubing, check valve, 185 cm 1/4" x 7/16" TPE thermoplastic tubing

CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag

spacer.gif

DHS+Frit

5:1

BRTF-

2000-C221

  • a (top-driven mixing parts): 3-blade impeller, diameter: 397 mm, angle: 45°

  • c (overlay): pressure sensor, ESG02MGSTKG12 (Saint-Gobain), 50 cm 1/2" x 3/4" TPE thermoplastic tubing + 30 cm 1/4" x 7/16"TPE thermoplastic tubing

  • f (feed): 50 cm 1/4" x 7/16" TPE thermoplastic tubing, 220cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • h, i (intlet'feed): 220 cm 1/4" x 3/4" TPE thermoplastic tubing, 50 cm 3/8" x 5/8" TPE thermoplastic tubing, plug

  • d (intlet'feed): 220 cm 1/4" x 7/16" TPE thermoplastic tubing, 50 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • g(Inlet): 15cm 1" x 1 - 3/8" TPE thermoplastic tubing, Y-connector(inner extension tubing)

    tubing 1: 15 cm 3/4" x 1"  TPE thermoplastic tubing, 250 cm 1/2" x 3/4" TPE thermoplastic tubing, plug

    tubing 2: 15 cm 3/4" x 1"  TPE thermoplastic tubing, 250 cm 1/2" x 3/4" TPE thermoplastic tubing, plug

  • b,j (vent filter): ESG02MGETTG12 (Saint-Gobain)×2, Y connector,

    tubing 1: 25 cm 3/4" x 1" TPE thermoplastic tubing

    tubing 2: 25 cm 3/4" x 1" TPE thermoplastic tubing

  • q (vent filter): 10 cm1/8" x1/4" TPE thermoplastic tubing,  Y-connector,

    tubing 1: 30 cm 1/8" x 1/4" TPE thermoplastic tubing, needless sampling

    tubing 2: 80 cm 1/8" x 1/4" TPE thermoplastic tubing, plug

  • r (sensor): thermowell, ID 3.5mm

  • x (harvest): 150 cm 3/4" x 1" TPE thermoplastic tubing, Y-connector, 

    tubing 1: 15 cm 3/4" x 1"  TPE thermoplastic tubing+ 60 cm 1/2" x 3/4" TPE thermoplastic tubing, plug

    tubing 2: 15 cm 3/4" x 1"  TPE thermoplastic tubing+ 60 cm 1/2" x 3/4" TPE thermoplastic tubing, plug

  • s,t,u,w (sparger): ESG02MGSTKG12 (Saint-Gobain), 8 cm 1/4" x 7/16" TPE thermoplastic tubing, check valve, 200 cm 1/4" x 7/16" TPE thermoplastic tubing

**Please be advised that this product is only available for sale and after-sales support in certain regions. For further details, kindly consult with your local sales representative. Thank you for your understanding.

Products

Link With Us Now!

Want to know more about BioLink bioprocessing products? Feel free to contact BioLink now!
Email
info@biolink.com
Phone
0086-400-058-9000
ADDRESS

Other Cell Culture Bag You May Like

CytoLinX<sup>®</sup> WB Single-use Cell Culture Bag CytoLinX® WB Single-use Cell Culture Bag
CytoLinX<sup>®</sup> WB Single-use Cell Therapy Bag CytoLinX® WB Single-use Cell Therapy Bag
CytoLinX<sup>®</sup> Single-use Breathable Cell Bag CytoLinX® Single-use Breathable Cell Bag
Wasted culture medium again? How to accurately determine the fermentation endpoint?
11 Feb 2026
Read More +
Wasted culture medium again? How to accurately determine the fermentation endpoint?
CytoLinX® Single-use Breathable Cell Bag: Ultra-low-density PE Film Enables Superior Gas Permeability
10 Feb 2026
Read More +
CytoLinX® Single-use Breathable Cell Bag: Ultra-low-density PE Film Enables Superior Gas Permeability
New Year Top Choice | BioLink SynaLinX® Pharmaceutical-Grade Tubing: Safeguarding the “Pulse” of Biopharmaceutical Processes
09 Feb 2026
Read More +
New Year Top Choice | BioLink SynaLinX® Pharmaceutical-Grade Tubing: Safeguarding the “Pulse” of Biopharmaceutical Processes
Download the Manuals
You authorize us to contact you and send you emails containing information about our services and products that may interests you. The authorization to process data for this purpose is not necessary, but you risk losing something that may interest you.
Products
Single-use Bioprocess Products Cell Culture Products Protein Chromatography Processing Filtration Hydration Products
Application & Solutions
Single-Use Bioprocess Solutions Monoclonal Antibody Production Vaccine Production mRNA Production
Resource
Application Note White Paper

Quick Links

Company Contact Us Validation Service FAQ
Contact Us
Email:
info@biolink.com
Newsletter
Copyright © Bio-Link All Rights Reserved.
Sitemap | Privacy Policy | 琼ICP备2023004837号-1
Products
Single-use Bioprocess Products Cell Culture Products Protein Chromatography Processing Filtration Hydration Products
Application & Solutions
Single-Use Bioprocess Solutions Monoclonal Antibody Production Vaccine Production mRNA Production
Resource
Application Note White Paper
Quick Links
Company Contact Us Validation Service FAQ
Contact Us
E-mail:
info@biolink.com Tel:
0086-400-058-9000
ADDRESS:
Copyright © Bio-Link All Rights Reserved.
Sitemap Privacy policy
We use cookies to offer you a better browsing experience, analyze site traffic and personalize content. By using this site, you agree to our use of cookies. Visit our cookie policy to learn more.
Reject Accept