VERDOT Ips² InPlace™ Process-scale Chromatography Column

Features of VERDOT Ips² InPlace™ Process-scale Chromatography Column

The VERDOT Ips² InPlace column is designed to be operated with a range of systems and accessories to enable:

• Cleaning in place of all wetted surfaces before column packing or unpacking

• Even distribution of the slurry over the column diameter during transfer

• Complete slurry transfer in syringe  mode using the motorized adaptor

• Self degassing before packing

• Reslurrying & unpacking in place with less than 2.5CV of buffer

• Cleaning in place of the column

• Maintenance in  place using the Column Lifter or optional Maintenance in place

• Closed system design

• Ideal for all media

• Variable height adaptor for  axial compression

• Supports multiple packing methods

• Fullly automated packing, unpacking & cleaning (optional)

• Reliable, sanitary and fast hydropneumatic   inflatable sealing system

• Low mechanical shear during media transfer

• Hoist-free operation   with optional Column Lifter or Maintenance in Place

• Complete: documentation for regulatory submissions

• Customizable: diameter up to 2.2m

Specifications of VERDOT Ips² InPlace™ Process-scale Chromatography Column

• Standard tube heights available: 600 and 900 mm.

• Columns with internal diameters >1,400 mm are available on request. 

• Higher pressure rating can be quoted on request.

• — Not available.

General Handling of VERDOT Ips² InPlace™ Process-scale Chromatography Column

Media transfer into the column can be achieved via syringe mode or external pump.

The low-shear valves are specially designed to minimize damage to chromatography media during transfer. The adaptor being free of retention self degas by just deflating the inflatable seal. The column is then axially compressed,  flow packed,  or both,  for optimal bed packing. The slurry valves can be rinsed while  the column is packed to prepare for unpacking.

Before the unpacking operation begins, the bed is reslurried in the upflow/downflow mode or by air sparging, while the piston is raised to its highest position. The slurry is then pumped out through the slurry valves, and when it is unloaded completely, the cleaning jets can be used to rinse the column.  The powerful jet and column tilting system help to ensure that no medium or residue remains on any contact surface and help to sanitize the column with less than 2.5 CV.

Variable Height Adaptor and Optional Packing Motor of VERDOT Ips² InPlace™ Process-scale Chromatography Column

VERDOT  Ips²  InPlace columns use a variable height adaptor with an adjustable central screw mechanism. This design fits any column diameter from pilot to manufacturing scale. The adaptor allows either axial compression or flow packing, or a combination of both, while the central screw mechanism allows simple yet precise adjustment of the bed height.

VERDOT  Ips²  offers optional packing motors for all column diameters.  A motor is strongly recommended for column diameters of  ≥296mm and is standard for columns  >1,000 mm.   The use of a  VERDOT   Ips² packing motor allows automatic movement (upward and downward) of the adaptor and controllable speed axial compression packing,   which makes packing reproducible, easier and faster.

Inflatable Seal of VERDOT Ips² InPlace™ Process-scale Chromatography Column

The design of the VERDOT  Ips² InPlace adaptor seal provides added security against leakage and inefficient packing by ensuring uniform pressure throughout the seal. All columns are equipped with an inflatable seal and hydropneumatic pump. The inflatable seal is filled with liquid  (NaOH or  EtOH)  and can be quickly inflated/deflated by using compressed air. Once inflated, the seal can be locked and isolated to permit unlimited storage of a packed column.

Regulatory Support and Quality Assurance of VERDOT Ips² InPlace™ Process-scale Chromatography Column

VERDOT    Ips²’s     manufacturing     procedures     mandate    full documentation of each step and every component in compliance with U.S. and European regulations. The original documentation and one electronic copy are included with each column. VERDOT Ips²   maintains    controlled   copies.    InPlace   chromatography columns   are   manufactured   in   compliance   with   engineering standards for pressure vessels, and each column comes with CE certification. The VERDOT Ips² manufacturing facility is certified according to ISO 9001:2008 standards.

• Manufacturing files for each project include:

• Engineering drawings

• Material traceability

• Weld report and welder qualifications

• Cleaning, electropolishing, and passivation certificates

• User manual and maintenance instructions

• Spare parts list

• Documentation for equipment from other vendors

• Factory acceptance test (FAT)

Systems & Accessories of VERDOT Ips² InPlace™ Process-scale Chromatography Column

A range of systems & accessories are available for column operation, each providing variable levels of automation:

1. Level  1: The Basic Control Console - controls packing motor speed.  The Transfer & Cleaning Device (TCD) both cleans and transfers the slurry in/out of the column. All controls are manual.

2. Level  2:  The  Advanced  Control  Console controls the packing motor speed/position and column pressure. Recipes/methods can be generated for automated

packing and unpacking operations.  The  Transfer  &  Cleaning Device (TCD) both cleans and transfers the slurry in/out of the column. All controls are manual.

3. Level 3: The Packing station - contains functional controls for automated slurry transfer, packing, unpacking and cleaning.  Ideal for use in dedicated “packing suites”.

4. Level 4: The All-in-one – all functionality as Level 3 + complete automation of the purification process.   Ideal for operations with limited space requirements.