New Product | FiltraLinX^® Benchtop Manual Hollow Fiber TFF System

FiltraLinX^® Benchtop Manual Hollow Fiber TFF System is an economical, practical, stable, and durable tangential flow filtration system that can meet the filtration area requirements from 0.017 to 5.1m², and is suitable for research and development stage, pilot-scale production, and GMP production.

FiltraLinX^® Application Scenarios

Fermentation liquid separation-Cell/bacteria separation
Clarification of lysate-Removal of cell/bacterial fragments
Purification of proteins, vaccines, viruses and plasmids
Diafiltration for culture solution, purification for blood products
Filtration for sterilization and virus removal

FiltraLinX^® Application Case

Hollow Fiber Harvest Clarification Experiment on Polysaccharide Lysis

Overview

A customer underwent lysis treatment after upstream harvesting and precipitation was generated. Considering the relatively longer processing time on using centrifugation and depth filtration for clarification and harvest, high requirements for the equipment of centrifugal harvest in a large scale harvest and high cost on processing and maintenance for the centrifugation equipment, the customer chose to use FiltraLinX^® Benchtop Manual Hollow Fiber TFF System and TFFNOVA^® Hollow Fiber for harvesting. Thus, this experiment was conducted to test the performance of the two products in this project.

Preparation for the experiment

1. Sample: Customer-provided sample of polysaccharide lysis

2. FiltraLinX^® Benchtop Manual Hollow Fiber TFF System

Model	Control method	Pump flow rate	Inner diameter of tube	Application point	Treatment volume
TS-HMV-Q03	Manual control	180 L/h	9.4mm	Polysaccharide clarification harvest	3.5L

3. TFFNOVA^® Hollow Fiber

Model

Aperture

Membrane Area

Batch Number

Application Point

Treatment Volume

S-20U-03C-080-N

0.2μm

1.0㎡

H230501A

Polysaccharide Clarification Harvest

3.5L

Experimental data

Time	Inlet Pressure	Backflow Pressure	Filtrate Pressure	Filtrate Data
Time	（bar）	（bar）	（bar）	Filtrate Data
11:25	0.2	0	0	Feed Volume: 1000ml
11:40	0.5	0.05	0	Processed Volume: 1500ml
11:55	0.6	0.1	0	Filtrate Volume: 100ml; Turbidity: 1.6NTU
11:55	0.6	0.1	0	Added Feed: 500ml; Processed Volume: 2000ml
12:10	0.6	0.1	0	Filtrate Volume: 100ml
12:25	0.6	0.1	0	Filtrate Volume: 100ml
12:40	0.6	0.1	0	Filtrate Volume: 100ml; Turbidity: 1.23NTU
13:03	0.8	0.2	0	Filtrate Volume: 300ml; Added Feed: 600ml
13:03	0.8	0.2	0	Processed Volume: 3500ml
13:15	1	0.6	0	Filtrate Volume: 200ml; Turbidity: 1.17NTU
13:30	1	0.8	0	Added 400ml Water for Diafiltration; Turbidity: 1.17NTU
13:50	1.5	1	0	Added 100ml Water for Diafiltration
13:55	1.5	1	0	Turbidity: 0.97NTU
14:30	Final processed harvest: 3.25L (estimated), Turbidity: 3.25NTU
14:30	Total Residual volume: 900ml, Turbidity: 3876NTU

Sample	Volume (ml)	Carbohydrates Content (mg/ml)	Total Amount (mg)
Initial	3500	8.817	30859.5
Final	3250	7.282	23665.5

From the experimental data, it can be analyzed that the FiltraLinX^® Benchtop Manual Hollow Fiber TFF System from BioLink and the TFFNOVA^® Hollow Fiber can be used for clarification of polysaccharide lysis. They are capable of harvesting the target product, and the polysaccharide lysis do not exhibit compatibility with the hollow fiber, and the yield meets customer's expectation.

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FiltraLinX® Application Case