Downstream Protein Chromatography Applications

Downstream bioprocessing refers to the later stages of the bioprocessing production process, which typically involve purifying and refining the bioprocessing product. Protein chromatography is a commonly used technique in downstream bioprocessing for purifying and refining proteins.

Protein Chromatography Working Principle

Protein chromatography works by selectively binding proteins to a stationary phase, typically a resin, based on specific interactions between the protein and the resin. The proteins can then be eluted from the resin, resulting in a purified and concentrated sample.

There are several different types of protein chromatography, including ion exchange chromatography, affinity chromatography, and hydrophobic interaction chromatography, each with specific applications and advantages.

Benefits of Protein Chromatography

The use of protein chromatography in downstream bioprocessing offers several benefits, including:

Improved product purity: Protein chromatography can remove impurities and contaminants, resulting in a highly purified protein product.

Increased yield: Protein chromatography can increase the yield of the bioprocessing product, as it enables the selective capture and purification of the target protein.

Increased efficiency: The use of protein chromatography can streamline the downstream bioprocessing process, reducing the time and cost associated with purifying and refining the bioprocessing product.

Popular Bioprocessing Products

Biohub^® Hose Plug

CNBr Activated Chromstar<sup>®</sup> Affinity Chromatography Resins

CNBr Activated Chromstar^® Affinity Chromatography Resins

BioHub^® Freeze-thaw Box

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Downstream Bioprocesses-the Role of Filtration

13 Feb 2023