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Headquartered in Shanghai, BioLink is a loyal partner of biopharmaceutical companies and institutions. By providing advanced and reliable products and solutions, we are committed to continuously promoting the rapid development of biotechnology industries such as antibodies, vaccines, recombinant protein and advanced therapies.

BioLink provides a range of bioprocessing products to biomanufacturers around the world, including cell culture, single-use biotechnology, and separation and purification products. We are committed to providing our customers with reliable bioprocess equipments and consumables that facilitate the long-term growth of the biomanufacturing field.

Application & Solutions

BioLink provides biopharmaceutical companies with specialized bioprocessing equipment for cell culture, protein chromatography, and processing filtration. Explore the role of bioprocess equipment and how BioLink supports the industry with reliable and flexible bioprocessing solutions.

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Hydrophobic Interaction Chromatography Resins

Hydrophobic Interaction Chromatography

In the downstream separation and purification process of biopharmaceuticals, chromatography is one of the indispensable separation methods. BioLink provides customers with 5 different chromatography solutions, including gel filtration chromatography, ion exchange chromatography, affinity chromatography, hydrophobic chromatography and multimodal chromatography. In addition, BioLink also produces chromatography columns and prepacked columns from laboratory to industrial grade. We focus on providing customers with high-quality products, professional solutions and comprehensive after-sales services.

Types of Hydrophobic Interaction Chromatography

MaXtar<sup>®</sup> Hydrophobic Chromatography Resins

Hydrophobic chromatography medium with high flow rate and high capacity.

MaXtar^® Hydrophobic Chromatography Resins

MaXtar<sup>®</sup> HR Hydrophobic Chromatography Resins

Hydrophobic chromatography media with high flow rate, high capacity and high resolution.

MaXtar^® HR Hydrophobic Chromatography Resins

Chromstar<sup>®</sup> FF Hydrophobic Chromatography Resins

Hydrophobic chromatography media with high flow rates.

Chromstar^® FF Hydrophobic Chromatography Resins

Chromstar<sup>®</sup> HP Hydrophobic Chromatography Resins

High Resolution Hydrophobic Chromatography Media.

Chromstar^® HP Hydrophobic Chromatography Resins

How To Select The Appropriate Chromatography Resin For My Own Process?

Since the hydrophobic characteristics of the target product are difficult to obtain directly, when designing a hydrophobic chromatography process, pre-experiments should be carried out with fillers containing different hydrophobic ligands. The hydrophobicity of the hydrophobic ligand butyl-octyl-phenyl increases sequentially, and the hydrophobicity of the target product can be predicted through the combined adsorption test with different hydrophobic fillers. After understanding the hydrophobicity of the target product, pretreatment of the target product should be carried out. Generally speaking, the target product will be combined with a hydrophobic filler in a high-salt system and eluted under a low-salt condition. Therefore, it is necessary to optimize the salt concentration during sample loading(ammonium sulfate is usually selected as the pretreatment salt for sample loading). In addition, after preliminarily determining the type of ligand and the salt concentration required for sample loading, the gradient elution from high salt to low salt can be used for filler screening and process optimization. The follow-up can be further optimized through experimental design.

Hydrophobic Chromatography Packing Suitable Samples

Hydrophobic chromatography is a chromatographic mode for purification based on the difference in hydrophobicity between the target product and impurities. Generally speaking, hydrophobic chromatography is mainly used for the removal of high molecular weight polymers.

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News & Events

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08 Nov 2024