info@biolink.com
+86-400-058-9000
CN
Support
FAQ Validation Service
Products
Application & Solutions
Single-Use Bioprocess Solutions Monoclonal Antibody Production Vaccine Production mRNA Production
Resource
Blog White Paper
Company
About Us Facilities Career Company News
Contact Us
Search
×
Single-Use Bioprocess Products Cell Culture Products Protein Chromatography Processing Filtration Hydration Products
Single-Use Bioprocess Bags and Bottles Single-Use Mixing System Aseptic Connect & Disconnect Bioprocess Supporting Vessels Aseptic Sampling Connectors and Fittings
BioHub®2D Single-Use Storage Bags BioHub® 3D Single-Use Storage Bags NovaLinX® Nuclease-free Single-Use Bioprocess Bags BioHub® Single-Use Storage Bottles BioHub® 3D Cubic Storage Bag BioHub® 3D Circular Storage Bag BioHub® Storage Bottle Tool Kit BioHub® BM Single-Use Magnetic Mixing System BioHub® DS Single-Use Split Desktop Mixing System BioHub® UM Open Top-Driven Mixing System BioHub® FD Feeding System BioHub® Single-Use Filling System BioHub® Single-Use Powder-Feeding Bag BioHub® MB Single-Use Mixing Bags BioHub® Single-Use Weighing Bag BioHub® Sealer BioHub® Welder BioHub® SC Sterile Cutter BioHub® 3D Circular Plastic Bin BioHub® 3D Cubic Foldable Plastic Bin BioHub® 3D Stainless Steel Storage System BioHub® Plastic Storage Tray BioHub® Cryopreservation Case BioHub® Stainless Steel Storage Tray BioHub® 2D Tray Transfer Cart BioHub® S Sterile Sampling Bags BioHub® S Sterile Sampling Bottles BioHub® SI Sterile Sampling Base BioHub® Connectors BioHub® Sanitary Clamp Fittings Biohub® Hose Plug BioHub® Diaphragm Gasket BioHub® Clamp & End Cap BioHub® Integrated Diaphragm Gauge Tee SynaLinX® B-Flex™ Thermoplastic Tubing SynaLinX® TST Platinum Cured Silicone Tubing CytoLinX® GB 1-20 L Benchtop Glass Bioreactors CytoLinX® BR 50-2000 L Single-Use Bioreactor CytoLinX® WB Single-Use Rocking Bioreactor CytoLinX® WB Single-Use Cell Therapy Bags CytoLinX® WB Single-Use Cell Culture Bags CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag BioHub® Magnetic Separator Chrom-LinX® Laboratory Scale Empty Chromatography Column Chrom-LinX® Manual Compression Chromatography Column Chrom-LinX® Auto Automated Chromatography Column PuriLinX® Benchtop Chromatography System VERDOT Ips² InPlace™ Process-scale Chromatography Column ChromaX ™ Geldex Prepacked Column Chrom-Trap® & Chrom-Screen® Prepacked Column ChromaX® A20 Affinity Chromatography Column Chromstar® FF Resin for Size Exclusion Chromatography Chromstar® CL Resin for Size Exclusion Chromatography Chromstar® B Resin for Size Exclusion Chromatography Geldex® PG Resin for Size Exclusion Chromatography Puredex® G Resin for Size Exclusion Chromatography Puredex® LH-20 Resin for Size Exclusion Chromatography Maxtar® Resins for Ion Exchange Chromatography Maxtar® HR Ion Exchange Chromatography MaXtar® HR XL Resins for Ion Exchange Chromatography Chromstar® BB Resins for Ion Exchange Chromatography Chromstar® FF Ion Exchange Chromatography Resins Chromstar® HP Ion Exchange Chromatography Resins Chromstar® XL Resins for Ion Exchange Chromatography Puredex® Ion Exchange Chromatography Resins MaXtar® ARPA Affinity Chromatography Resins MaXtar® rProtein A Affinity Chromatography Resins MaXtar® Protein G Affinity Chromatography Resins rProtein A Chromstar® FF Affinity Chromatography Resins Protein G Chromstar® 4FF Affinity Chromatography Resins Protein G Chromstar® HP Affinity Chromatography Resins Ni Chromstar® Affinity Chromatography Resins IMAC Chromstar® Affinity Chromatography Resins MaXtar® Chelating Affinity Chromatography Resins Chelating Chromstar® FF Affinity Chromatography Resins Glutathione Chromstar® Affinity Chromatography Resins MaXtar® PlasmidCap HR Affinity Chromatography Resins PlasmidCap Chromstar® HP Affinity Chromatography Resins MaXtar® Heparin Affinity Chromatography Resins Heparin Chromstar® Affinity Chromatography Resins Benzamidine Chromstar® 4FF Affinity Chromatography Resins MaXtar® Blue Affinity Chromatography Resins Blue Chromstar® Affinity Chromatography Resins NHS Activated Chromstar® 4FF Affinity Chromatography Resins CNBr Activated Chromstar® Affinity Chromatography Resins MaXtar® Hydrophobic Chromatography Resins MaXtar® HR Hydrophobic Chromatography Resins Chromstar® FF Hydrophobic Chromatography Resins Chromstar® HP Hydrophobic Chromatography Resins MaXtar® MMC Multimodal Chromatography Resins Maxtar® COLL Multimodal Chromatography Resins Maxtar® MMA Multimodal Chromatography Resins Hydroxyapatite BARONHAP™ Type I&2 Multimodal Chromatography Resin Maxgo® 15S Polymer Chromatography FiltraLinX® Depth Filter Holder (Type P3C) Depth Filtration Bioprocess Fixture Type M FiltraLinX® Virus Filter Holder FiltraLinX® Tangential Flow Filtration Holder TFFNOVA® Hollow Fiber Filter FiltraLinX® Benchtop Manual Hollow Fiber TFF System FiltraLinX® Benchtop Semi-Automatic TFF System FiltraLinX® Benchtop TFF System HydroLinX® Water for Injection in Bottles HydroLinX® Water for Injection in Bags HydroLinX® Buffer Solutions and Process Liquids HydroLinX® Antifoaming Agent
E-mail

CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag

The core of the CytoLinX® BRCF 50 L microbial fermentation system is the single-use microbial bioreactor bag designed to meet the stringent requirements of microbial fermentation. It is used for cultivating various organisms, including E-coli, pseudomonas, and yeast. The single-use microbial bioreactor bag is based on the proven design and materials of the CytoLinX® BRCF Single-Use Bioreactor Bag for mammalian cell culture.

  • RENOLIT 9101 multilayer co-extruded film, ULDPE liquid contact layer, offering excellent biocompatibility and chemical compatibility while ensuring low levels of extractable content.

  • The dual impeller design enables vigorous mixing of the culture, and the bottom magnetic coupling eliminates external shafts, minimizing the risk of leakage.

  • All single-use microbial bioreactor bags are equipped with pressure sensors to maintain bag integrity during demanding fermentation processes.

  • The vent filter is equipped with a condensation bag at the front end to integrate condensation, returning water vapor from the gas back into the bag, ensuring the ventfilter remains unclogged.

Inquiry Download
Features Specifications Contact us

Features of CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag

CytoLinX® BRTF Single-Use Top-Driven Bioreactor Bag is designed to match single-use top mechanical coupling bioreactor used in biopharmaceuticals. The product can be used for scientific research, process development and commercial production of CHO, Vero, and MDCK cells, etc.

  • RENOLIT 9101 multilayer co-extruded film, ULDPE fluid contact layer, offering excellent biocompatibility and chemical compatibility while ensuring low levels of extractable content

  • The porous-frit microsparge column is designed from ultra-high molecular weight polyethylene (UHMW- PE), with pore sizes ranging from 20-40 μm. The generated bubbles possess a high surface area ratio and good oxygen transfer. UHMW-PE exhibits outstanding impact resistance, wear resistance, chemical corrosion resistance, physiological inertness, adaptability, and hydrophobicity

  • The macro-perforated microporous membrane is a dispersed aeration disc based on film. Laser-drilled to maintain uniform pore size, various specifications such as 0.178mm, 0.233mm, 0.368mm, 0.445mm, 0.582mm are available, tailored with specific apertures and quantities for each bag specification

  • Equipped with imported filters to ensure bag integrity

  • All pipelines can be flexibly customized

  • Fully validated, complete validation reports can be provided


Specifications of CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag

Standard Configuration of CytoLinX® BRCF Single-Use Bottom-Driven Microbial Bioreactor Bag

Volume

Version

Cat. No.

Configuration







CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag












Medium sparge












BRCF-

0050-P404

  • Min. working volume: 15 L

  • Max. working volume: 50 L

  • Impeller: double-layer, 6 Rushton blades, pitch blade at the top, axial flow impeller,diameter: 195 mm, bottom-driven centric mixing

  • a，b，e (feeding port): 05 cm 1/8" x 1/4" C-Flex, hose plug

  • c (pressure monitoring): pressure sensor, 45 cm 1/2" x 3/4" C-Flex, hose plug

  • d (vent filter + condensation bag): 37 cm 1" x 1-3/8" C-Flex，condensation bag，20 cm 1" x 1-3/8"C-Flex，L10SSAPBBG1P， 35 cm 1" x 1-3/8" C-Flex，L05SSAPBBG1P

  • f (feeding port): 205 cm 1/8" x 1/4" C-Flex(×2）, hose plug(×2）

  • g (spare vent filter inlet）:30 cm 1" x 1-3/8" C-Flex , AseptiQuik® L sterile connector；

  • h (intlet): 205 cm 3/8" x 5/8" C-Flex, hose plug

  • i (sampling port): 50 cm 1/8" x 1/4" C-Flex(× 2), sterile sampling valve (× 2）

  • j，k (sensor): Kleenpak ™ sterile connector female adapter

  • l (sensor): thermowell, ID3.5 mm

  • 1，3，5 (harvest tubing): 128 cm 3/8" x 5/8" C-Flex, hose plug, OD1/8"-OD1" pinch valve

  • 2，4 , 6 (bottom gas): CL2VTV0.2-002 (Meissner), 168 cm 1/4" x 7/16"C-Flex， TC 25 + 6 # quick plug for gas tubing（Only one filter for pure medium sparge）


Standard Configuration of CytoLinX® BRTF Single-Use Bottom-Driven Bioreactor Bag

Volume

Version

Cat. No.

Configuration






CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag

Micro + 

Macro 

sparge 5:1


BRTF-

0050-C201

  • a（top-driven mixing parts)：3-blade impeller，diameter：111.1 mm， angle: 45°

  • b（headspace gas）：pressure sensor, CF2VTV0.2-33B1 (Meissner),20 cm1/2" x 3/4" C-Flex + 16 cm 1/4" x 7/16"C-Flex，6 # quick plug for gas tubing

  • c（intlet / feeding port）：150 cm 1/4" x 7/16" C-Flex，30 cm 1/8" x 1/4" C-Flex plug

  • k（intlet / feeding port）：150 cm 3/8" x 5/8" C-Flex，Y connector，

  • tubing 1：40 cm 3/8" x 5/8" C-Flex，plug；

  • tubing 2：10 cm 3/8" x 5/8" C-Flex，30 cm 1/4" x 7/16" C-Flex，plug

  • l（intlet）：180 cm 3/8" x 5/8" C-Flex，plug

  • n（feeding port）：15 cm 1/4" x 7/16" C-Flex，150 cm 1/8" x 1/4" C-Flex，plug

  • m（vent filter）：CS2VTV0.2-002 (Meissner), Y connector，

  • tubing 1：25 cm 1/2" x 3/4" C-Flex；tubing 2：15 cm 1/2" x 3/4" C-Flex，AseptiQuik® G sterile connector，1/2"HB

  • d,e,f,g( sensor ): AseptiQuik® G sterile connector，1/2"HB

  • h（sampling port）：30 cm 1/8" x 1/4" C-Flex，needless sampling 50 cm 1/8" x 1/4" C-Flex，plug

  • i（sensor）：thermowell，ID3.5 mm

  • j（harvest port）：100 cm 1/2" x 3/4" C-Flex，30 cm 3/8" x 5/8" C-Flex， plug

  • o,p(bottom gas)：CF2VTV0.2-33B1 (Meissner)，15 cm 1/4" x 7/16"C-Flex， one-way valve，150 cm 1/4" x 7/16" C-Flex，6 # quick plug for tubing

Micro + 

Marco

sparge 2:1


BRTF-

0050-C202



Volume

Version

Cat. No.

Configuration





CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag


Micro + Macro

sparge 5:1


BRTF-

0250-C201

  • a (top-driven mixing parts)：3-blade impeller，diameter：251 mm， angle: 45°

  • i (headspace gas)：pressure sensor , CS2VTV0.2-002 (Meissner),

  • 20 cm1/2" x 3/4" C-Flex + 16 cm 1/4" x 7/16"C-Flex， 6# quick plug for gas tubing

  • m (feeding port)：15 cm 1/4" x 7/16" C-Flex，180 cm 1/8" x 1/4" C-Flex， plug

  • k,l（intlet / feeding port)：150 cm 3/8" x 5/8" C-Flex，Y connector，

    tubing 1：30 cm 3/8" x 5/8" C-Flex，plug；

    tubing 2：20 cm 3/8" x 5/8" C-Flex，40 cm 1/8" x 1/4" C-Flex，plug

  • n (intlet / feeding port)：150 cm 1/4" x 7/16" C-Flex，50 cm 1/8" x 1/4" C-Flex， plug

  • j (vent filter)：CL2VTV0.2-002 (Meissner), Y connector，

    tubing 1：30 cm 1/2" x 3/4" C-Flex；

    tubing 2 ：20 cm 1/2" x 3/4" C-Flex，AseptiQuik® G sterile connector， 1/2"HB

  • b,c,d,e (sensor)：AseptiQuik® G sterile connector，1/2"HB

  • g (sampling port)：30 cm 1/8" x 1/4" C-Flex，needless sampling；

  • 50 cm 1/8" x 1/4" C-Flex，plug

  • f (sensor)：thermowell，ID3.5 mm

  • h (harvest port)：150 cm 1/2" x 3/4" C-Flex，plug

  • o,p (bottom gas) ：CF2VTV0.2-33B1 (Meissner)，15 cm 1/4" x 7/16"C-Flex， one-way valve，150 cm 1/4" x 7/16" C-Flex，6# quick plug for gas tubing





Micro + Marco

sparge 2:1






BRTF-

0250-C202



CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag







Micro + Macro

sparge 5:1







BRTF-

0500-C201

  • a (top-driven mixing parts)：3-blade impeller，diameter：251 mm， angle: 45°

  • k (headspace gas)：pressure sensor , CS2VTV0.2-002 (Meissner),

  • 25 cm 1/2" x 3/4" C-Flex + 16 cm 1/4" x 7/16"C-Flex，6# quick plug for gas tubing

  • l（feeding port）： 15 cm 1/4" x 7/16" C-Flex，230 cm 1/8" x 1/4" C-Flex， plug

  • m (intlet / feeding port)：250 cm 1/4" x 7/16" C-Flex，plug，

  • n,o (intlet / feeding port)：180 cm 3/8" x 5/8" C-Flex，connector

    tubing 1：30 cm 3/8" x 5/8" C-Flex，plug；

    tubing 2：30 cm 3/8" x 5/8" C-Flex，30 cm 1/8" x 1/4" C-Flex，plug

  • p (intlet / feeding port)：250 cm 3/8" x 5/8" C-Flex，plug

  • j (vent filter)：CUVTV0.2-1N002 (Meissner), connector， tubing 1：30 cm 3/4" x 1" C-Flex；tubing 2：20 cm 3/4" x 1" C-Flex，AseptiQuik® G sterile connector， 1/2"HB

  • b,c,d,e,f（sensor）：AseptiQuik® G sterile connector，1/2"HB

  • g (sampling port）：30 cm 1/8" x 1/4" C-Flex，needless sampling；80 cm 1/8" x 1/4" C-Flex，plug

  • h（sensor）：thermowell，ID3.5 mm

  • i（harvest port）：150 cm 1/2" x 3/4" C-Flex，plug

  • q，r (bottom gas）：CS2VTV0.2-002 (Meissner)，16 cm 1/4" x 7/16"C-Flex， one-way valve，185 cm 1/4" x 7/16" C-Flex，6# quick plug for gas tubing


Volume

Version

Cat. No.

Configuration








CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag








Micro + Macro

sparge 5:1








BRTF-

1000-C201

  • a (top-driven mixing parts）：3-blade impeller，diameter：321 mm， angle :45°

  • k（headspace gas）：pressure sensor , CS2VTV0.2-002 (Meissner), 50 cm1/2" x 3/4" C-Flex + 20 cm 1/4" x 7/16"C-Flex， 6# quick plug for gas tubing

  • l（feeding port）： 15 cm 1/4" x 7/16" C-Flex，250 cm 1/8" x 1/4" C-Flex， plug

  • m (intlet / feeding port）：250 cm 1/4" x 7/16" C-Flex，30 cm 1/8" x 1/4" C-Flex，plug

  • n,o (intlet / feeding port）：250 cm 3/8" x 5/8" C-Flex，connector， tubing 1：30 cm 3/8" x 5/8" C-Flex，plug；tubing 2：20 cm 3/8" x 5/8" C-Flex，35 cm 1/8" x 1/4" C-Flex，plug

  • j（vent filter）：CUVTV0.2-1N002 (Meissner)×2, connector， tubing 1：25 cm 3/4" x 1" C-Flex；tubing 2：25 cm 3/4" x 1" C-Flex

  • b,c,d,e,f（sensor）：AseptiQuik® G sterile connector，1/2"HB

  • g（sampling port）：30 cm 1/8" x 1/4" C-Flex，needless sampling；80 cm 1/8" x 1/4" C-Flex，plug

  • h（sensor）：thermowell，ID3.5 mm

  • i（harvest port）：160 cm 1/2" x 3/4" C-Flex，30 cm 3/8" x 5/8" C-Flex， connector

    tubing 1：30 cm 3/8" x 5/8" C-Flex，plug；

    tubing 2：20 cm 3/8" x 5/8" C-Flex，35 cm 1/8" x 1/4" C-Flex，plug • p，q（bottom gas）：CS2VTV0.2-002 (Meissner)，16 cm

  • 1/4" x 7/16"C-Flex，one-way valve，185 cm 1/4" x 7/16" C-Flex，6# quick plug for gas tubing








CytoLinX® BRCF Single-Use Top-Driven Bioreactor Bag

spacer.gif







Micro + Macro

sparge 5:1







BRTF-

2000-C201

  • a（top-driven mixing parts）：3-blade impeller，diameter：397 mm， angle: 45°

  • c（headspace gas）：pressure sensor , CS2VTV0.2-002 (Meissner), 50 cm 1/2" x 3/4" C-Flex + 30 cm 1/4" x 7/16"C-Flex，6# quick plug for gas tubing

  • d，g（ifeeding port）：50 cm 1/4" x 7/16" C-Flex，220 cm 1/8" x 1/4" C-Flex， plug

  • e (intlet / feeding port)：220 cm 1/2" x 3/4" C-Flex，50 cm 3/8" x 5/8" C-Flex， plug

  • f (intlet / feeding port)：220 cm 1/4" x 7/16" C-Flex，50 cm 1/8" x 1/4" C-Flex， plug

  • h (intlet)：15 cm 1" x 1 - 3/8" C-Flex，connector (internal extended tube)，

    tubing 1：15 cm 3/4" x 1" C-Flex，250 cm 1/2" x 3/4" C-Flex，plug；

    tubing 2：15 cm 3/4" x 1" C-Flex，250 cm 1/2" x 3/4" C-Flex，plug

  • b (vent filter) ：CUVTV0.2-1N002 (Meissner)×2, Y connector，

    tubing 1：30 cm 3/4" x 1" C-Flex；

    tubing 2：30 cm 3/4" x 1" C-Flex

  • i,j,k,l,m (sensor) ：AseptiQuik® G sterile connector，1/2"HB

  • n (sampling port) ：30 cm 1/8" x 1/4" C-Flex，needless sampling；80 cm 1/8" x 1/4" C-Flex，plug

  • o（sensor）：thermowell，ID3.5 mm

  • p（harvest port）：200 cm 3/4" x 1" C-Flex，TC 50

  • q，r，s，t（bottom gas）：CS2VTV0.2-002 (Meissner)，8 cm1/4" x7/16" C-Flex，one-way valve，200 cm 1/4" x 7/16" C-Flex， 6# quick plug for gas tubing


Products

Link With Us Now!

Want to know more about BioLink bioprocessing products? Feel free to contact BioLink now!
Email
info@biolink.com
Phone
+86-400-058-9000
ADDRESS

Other Cell Culture Bag You May Like

CytoLinX<sup>®</sup> WB Single-Use Cell Therapy Bags CytoLinX® WB Single-Use Cell Therapy Bags
CytoLinX<sup>®</sup> WB Single-Use Cell Culture Bags CytoLinX® WB Single-Use Cell Culture Bags
News & Events
New Product | BioLink CytoLinX GB Benchtop Glass Bioreactor
20 Jul 2024
Read More +
New Product | BioLink CytoLinX GB Benchtop Glass Bioreactor
BioLink Commercialization Scale Chromatography Resins Change Case Sharing
10 Jul 2024
Read More +
BioLink Commercialization Scale Chromatography Resins Change Case Sharing
Guide to Choosing CytoLinX®BR Disposable Bioreactor Bags
01 Jul 2024
Read More +
Guide to Choosing CytoLinX®BR Disposable Bioreactor Bags
Products
Single-Use Bioprocess Products Cell Culture Products Protein Chromatography Processing Filtration Hydration Products
Application & Solutions
Single-Use Bioprocess Solutions Monoclonal Antibody Production Vaccine Production mRNA Production
Resource
Blog White Paper

Quick Links

Company Contact Us Validation Service FAQ
Contact Us
Email:
info@biolink.com
Newsletter
Copyright © Bio-Link All Rights Reserved.
Sitemap | Privacy Policy | 琼ICP备2023004837号-1
Products
Single-Use Bioprocess Products Cell Culture Products Protein Chromatography Processing Filtration Hydration Products
Application & Solutions
Single-Use Bioprocess Solutions Monoclonal Antibody Production Vaccine Production mRNA Production
Resource
Blog White Paper
Quick Links
Company Contact Us Validation Service FAQ
Contact Us
E-mail:
info@biolink.com Tel:
+86-400-058-9000
ADDRESS:
Copyright © Bio-Link All Rights Reserved.
Sitemap Privacy policy
We use cookies to offer you a better browsing experience, analyze site traffic and personalize content. By using this site, you agree to our use of cookies. Visit our cookie policy to learn more.
Reject Accept