Chelating Chromstar® FF is a metal chelate affinity chromatography resin made by covalently cross-linking iminodiacetic acid (IDA) to the Chromstar® matrix via a spacer arm. IDA provides 3 ligand sites for chelation with transition metal ions (Cu2+, Zn2+, Co2+, Ni2+, etc.). The chromatography resin exploits the interaction between transition metal ions and histidine, cysteine, tryptophan, etc., which are widely present in proteins to separate and purify proteins with and without these amino acids and with high or low amounts of these amino acids.
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